10+ puasp-attb vector
When the pUASTattB and the φC31 helper plasmids are co-injected into cells containing attP. To recombine an Entry clone with a Destination clone.
Common Vectors
Gateway cloning using pENTR-D-AthHEN1DmeCO entry vector and pUASp-FMW-attB destination vector backbone_origin.
. Miniprep DNA of the desired Destination vector. For this reason the pUASTattB vector system is designed to be used with Drosophila lines carrying attP landing sites within their genome. Sutter Instrument transformation vector puasp k10 attb Transformation Vector Puasp K10 Attb supplied by Sutter Instrument used in various techniques.
Zhang Z Xu J Koppetsch B Wang J Tipping C Ma S et alHeterotypic piRNA Ping-Pong requires qin a protein with both E3 ligase and Tudor domains. Stefan Ameress lab contains the insert Hen1 and is published in Nat Methods. Miniprep DNA of an Entry clone containing your ORF.
SnapGene Viewer is free software that allows molecular biologists to create browse and share richly annotated sequence files. Gateway LR Clonase Enzyme Mix. Each CI candidate gene cinA cinB cinB-K636A and cinA-T2A-cinB was inserted into the pUASp-attB vector by standard cloning techniques without codon optimization.
Plasmid pUASp-FMW-attB-AthHen1 from Dr. Gain unparalleled visibility of your plasmids DNA and protein. The other vector referred to as the helper plasmid encodes for the φC31 integrase.
86100 based on 1. Search by vector name or feature using the product search above or browse the vectors through the category drop menu down below. Search by vector name or feature using the product search above or browse the vectors through the category drop menu down below.
The Drosophila Gateway Vector Collection Department Of Embryology
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